Chromatin Immunoprecipitation:Article Title: Selective utilization of glucose metabolism guides mammalian gastrulation
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Glut1 Ms 1:250 MA1-37783 (Invitrogen) Snai1 Gt 1:200 AF3639 (R&D Systems) Glut3 Rb 1:250 MA532696 (Invitrogen) NCad Ms 1:200 MA1-91128 (Invitrogen) Sox2 Rt 1:800 14-9811-82 (Invitrogen) T/Bra Gt 1:500, 1:200 AF2085 (R&D Systems) T/Bra Rb 1:250 81694S (Cell Signaling) Lef1 Rb 1:400 MA5-14966 (Invitrogen) Laminin Rb 1:400 PA1-16730 (Invitrogen) Mmp14 Rb 1:250 PA5-13183 (Invitrogen) Ecad Rt 1:200 13-1900 (Invitrogen) dpErk Rb 1:100 4377T (Cell Signaling) P-Histone H3 Rb 1:500 441190G (Invitrogen) Foxa2 Rb 1:200 8186S (Cell Signalling) Eomes Rt 1:200 53-4875-80 (Invitrogen) Syndecan-1 Rb 1:300 PA516918 (Invitrogen) HS Chain 10E4 Ms 1:500 Clone F58, 37255-5 (Amsbio) ERK1/2 (Western Blot) Rb 1:1000 4695 (Cell Signalling) pERK (Western Blot) Rb 1:1000 9101 (Cell Signalling) Lamin B Ms 1:1000 ab8982 (Abcam) MitoTracker Deep Red - 1:800 M7512 (Invitrogen) TMRM - 1:800 T668 (Invitrogen) DAPI 405 1:500 D3571 F-Actin (647) 647 1:200 A22287 F-Actin (488) 488 1:500 A12379 donkey anti-mouse Alexa Fluor 488 488 1:500 A21202 donkey anti-rabbit Alexa Fluor 488 488 1:500 A21206 donkey anti-rat Alexa Fluor 488 488 1:500 A21208 donkey anti-mouse Alexa Fluor 568 568 1:500 A10039 donkey anti-goat Alexa Fluor 568 568 1:500 A11057 donkey anti-rabbit Alexa Fluor 568 568 1:500 A10042 donkey anti-mouse Alexa Fluor 647 647 1:500 A31571 donkey anti-goat Alexa Fluor 647 647 1:500 A21447 donkey anti-rabbit Alexa Fluor 647 647 1:500 A31573 Anti-rabbit IgG, HRP-linked Antibody HRP 1:5000 7074 Anti-mouse IgG, HRP-linked Antibody HRP 1:5000 7076 Validation The subcellular localization of all the proteins analyzed in this study has been previously reported in mouse embryo (shown as immunofluorescence labelling). .. Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) The ERK-KTR mESCs used in this study source from Simon et al (JAX#035333).
Flow Cytometry:Article Title: Selective utilization of glucose metabolism guides mammalian gastrulation
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Glut1 Ms 1:250 MA1-37783 (Invitrogen) Snai1 Gt 1:200 AF3639 (R&D Systems) Glut3 Rb 1:250 MA532696 (Invitrogen) NCad Ms 1:200 MA1-91128 (Invitrogen) Sox2 Rt 1:800 14-9811-82 (Invitrogen) T/Bra Gt 1:500, 1:200 AF2085 (R&D Systems) T/Bra Rb 1:250 81694S (Cell Signaling) Lef1 Rb 1:400 MA5-14966 (Invitrogen) Laminin Rb 1:400 PA1-16730 (Invitrogen) Mmp14 Rb 1:250 PA5-13183 (Invitrogen) Ecad Rt 1:200 13-1900 (Invitrogen) dpErk Rb 1:100 4377T (Cell Signaling) P-Histone H3 Rb 1:500 441190G (Invitrogen) Foxa2 Rb 1:200 8186S (Cell Signalling) Eomes Rt 1:200 53-4875-80 (Invitrogen) Syndecan-1 Rb 1:300 PA516918 (Invitrogen) HS Chain 10E4 Ms 1:500 Clone F58, 37255-5 (Amsbio) ERK1/2 (Western Blot) Rb 1:1000 4695 (Cell Signalling) pERK (Western Blot) Rb 1:1000 9101 (Cell Signalling) Lamin B Ms 1:1000 ab8982 (Abcam) MitoTracker Deep Red - 1:800 M7512 (Invitrogen) TMRM - 1:800 T668 (Invitrogen) DAPI 405 1:500 D3571 F-Actin (647) 647 1:200 A22287 F-Actin (488) 488 1:500 A12379 donkey anti-mouse Alexa Fluor 488 488 1:500 A21202 donkey anti-rabbit Alexa Fluor 488 488 1:500 A21206 donkey anti-rat Alexa Fluor 488 488 1:500 A21208 donkey anti-mouse Alexa Fluor 568 568 1:500 A10039 donkey anti-goat Alexa Fluor 568 568 1:500 A11057 donkey anti-rabbit Alexa Fluor 568 568 1:500 A10042 donkey anti-mouse Alexa Fluor 647 647 1:500 A31571 donkey anti-goat Alexa Fluor 647 647 1:500 A21447 donkey anti-rabbit Alexa Fluor 647 647 1:500 A31573 Anti-rabbit IgG, HRP-linked Antibody HRP 1:5000 7074 Anti-mouse IgG, HRP-linked Antibody HRP 1:5000 7076 Validation The subcellular localization of all the proteins analyzed in this study has been previously reported in mouse embryo (shown as immunofluorescence labelling). .. Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) The ERK-KTR mESCs used in this study source from Simon et al (JAX#035333).
Magnetic Resonance Imaging:Article Title: Selective utilization of glucose metabolism guides mammalian gastrulation
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Glut1 Ms 1:250 MA1-37783 (Invitrogen) Snai1 Gt 1:200 AF3639 (R&D Systems) Glut3 Rb 1:250 MA532696 (Invitrogen) NCad Ms 1:200 MA1-91128 (Invitrogen) Sox2 Rt 1:800 14-9811-82 (Invitrogen) T/Bra Gt 1:500, 1:200 AF2085 (R&D Systems) T/Bra Rb 1:250 81694S (Cell Signaling) Lef1 Rb 1:400 MA5-14966 (Invitrogen) Laminin Rb 1:400 PA1-16730 (Invitrogen) Mmp14 Rb 1:250 PA5-13183 (Invitrogen) Ecad Rt 1:200 13-1900 (Invitrogen) dpErk Rb 1:100 4377T (Cell Signaling) P-Histone H3 Rb 1:500 441190G (Invitrogen) Foxa2 Rb 1:200 8186S (Cell Signalling) Eomes Rt 1:200 53-4875-80 (Invitrogen) Syndecan-1 Rb 1:300 PA516918 (Invitrogen) HS Chain 10E4 Ms 1:500 Clone F58, 37255-5 (Amsbio) ERK1/2 (Western Blot) Rb 1:1000 4695 (Cell Signalling) pERK (Western Blot) Rb 1:1000 9101 (Cell Signalling) Lamin B Ms 1:1000 ab8982 (Abcam) MitoTracker Deep Red - 1:800 M7512 (Invitrogen) TMRM - 1:800 T668 (Invitrogen) DAPI 405 1:500 D3571 F-Actin (647) 647 1:200 A22287 F-Actin (488) 488 1:500 A12379 donkey anti-mouse Alexa Fluor 488 488 1:500 A21202 donkey anti-rabbit Alexa Fluor 488 488 1:500 A21206 donkey anti-rat Alexa Fluor 488 488 1:500 A21208 donkey anti-mouse Alexa Fluor 568 568 1:500 A10039 donkey anti-goat Alexa Fluor 568 568 1:500 A11057 donkey anti-rabbit Alexa Fluor 568 568 1:500 A10042 donkey anti-mouse Alexa Fluor 647 647 1:500 A31571 donkey anti-goat Alexa Fluor 647 647 1:500 A21447 donkey anti-rabbit Alexa Fluor 647 647 1:500 A31573 Anti-rabbit IgG, HRP-linked Antibody HRP 1:5000 7074 Anti-mouse IgG, HRP-linked Antibody HRP 1:5000 7076 Validation The subcellular localization of all the proteins analyzed in this study has been previously reported in mouse embryo (shown as immunofluorescence labelling). .. Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) The ERK-KTR mESCs used in this study source from Simon et al (JAX#035333).
Western Blot:Article Title: Selective utilization of glucose metabolism guides mammalian gastrulation
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Glut1 Ms 1:250 MA1-37783 (Invitrogen) Snai1 Gt 1:200 AF3639 (R&D Systems) Glut3 Rb 1:250 MA532696 (Invitrogen) NCad Ms 1:200 MA1-91128 (Invitrogen) Sox2 Rt 1:800 14-9811-82 (Invitrogen) T/Bra Gt 1:500, 1:200 AF2085 (R&D Systems) T/Bra Rb 1:250 81694S (Cell Signaling) Lef1 Rb 1:400 MA5-14966 (Invitrogen) Laminin Rb 1:400 PA1-16730 (Invitrogen) Mmp14 Rb 1:250 PA5-13183 (Invitrogen) Ecad Rt 1:200 13-1900 (Invitrogen) dpErk Rb 1:100 4377T (Cell Signaling) P-Histone H3 Rb 1:500 441190G (Invitrogen) Foxa2 Rb 1:200 8186S (Cell Signalling) Eomes Rt 1:200 53-4875-80 (Invitrogen) Syndecan-1 Rb 1:300 PA516918 (Invitrogen) HS Chain 10E4 Ms 1:500 Clone F58, 37255-5 (Amsbio) ERK1/2 (Western Blot) Rb 1:1000 4695 (Cell Signalling) pERK (Western Blot) Rb 1:1000 9101 (Cell Signalling) Lamin B Ms 1:1000 ab8982 (Abcam) MitoTracker Deep Red - 1:800 M7512 (Invitrogen) TMRM - 1:800 T668 (Invitrogen) DAPI 405 1:500 D3571 F-Actin (647) 647 1:200 A22287 F-Actin (488) 488 1:500 A12379 donkey anti-mouse Alexa Fluor 488 488 1:500 A21202 donkey anti-rabbit Alexa Fluor 488 488 1:500 A21206 donkey anti-rat Alexa Fluor 488 488 1:500 A21208 donkey anti-mouse Alexa Fluor 568 568 1:500 A10039 donkey anti-goat Alexa Fluor 568 568 1:500 A11057 donkey anti-rabbit Alexa Fluor 568 568 1:500 A10042 donkey anti-mouse Alexa Fluor 647 647 1:500 A31571 donkey anti-goat Alexa Fluor 647 647 1:500 A21447 donkey anti-rabbit Alexa Fluor 647 647 1:500 A31573 Anti-rabbit IgG, HRP-linked Antibody HRP 1:5000 7074 Anti-mouse IgG, HRP-linked Antibody HRP 1:5000 7076 Validation The subcellular localization of all the proteins analyzed in this study has been previously reported in mouse embryo (shown as immunofluorescence labelling). .. Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) The ERK-KTR mESCs used in this study source from Simon et al (JAX#035333).
Article Title: Selective utilization of glucose metabolism guides mammalian gastrulation
Article Snippet: .. Antibody Host species Dilution Catalog number Glut1 Ms 1:250 MA1-37783 (Invitrogen) Snai1 Gt 1:200 AF3639 (R&D Systems) Glut3 Rb 1:250 MA532696 (Invitrogen) NCad Ms 1:200 MA1-91128 (Invitrogen) Sox2 Rt 1:800 14-9811-82 (Invitrogen) T/Bra Gt 1:500, 1:200 AF2085 (R&D Systems) T/Bra Rb 1:250 81694S (Cell Signaling) Lef1 Rb 1:400 MA5-14966 (Invitrogen) Laminin Rb 1:400 PA1-16730 (Invitrogen) Mmp14 Rb 1:250 PA5-13183 (Invitrogen) Ecad Rt 1:200 13-1900 (Invitrogen) dpErk Rb 1:100 4377T (Cell Signaling) P-Histone H3 Rb 1:500 441190G (Invitrogen) Foxa2 Rb 1:200 8186S (Cell Signalling) Eomes Rt 1:200 53-4875-80 (Invitrogen) Syndecan-1 Rb 1:300 PA516918 (Invitrogen) HS Chain 10E4 Ms 1:500 Clone F58, 37255-5 (Amsbio) ERK1/2 (Western Blot) Rb 1:1000 4695 (Cell Signalling) pERK (Western Blot) Rb 1:1000 9101 (Cell Signalling) Lamin B Ms 1:1000 ab8982 (Abcam) MitoTracker Deep Red - 1:800 M7512 (Invitrogen) TMRM - 1:800 T668 (Invitrogen) ..
Biomarker Discovery:Article Title: Selective utilization of glucose metabolism guides mammalian gastrulation
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Glut1 Ms 1:250 MA1-37783 (Invitrogen) Snai1 Gt 1:200 AF3639 (R&D Systems) Glut3 Rb 1:250 MA532696 (Invitrogen) NCad Ms 1:200 MA1-91128 (Invitrogen) Sox2 Rt 1:800 14-9811-82 (Invitrogen) T/Bra Gt 1:500, 1:200 AF2085 (R&D Systems) T/Bra Rb 1:250 81694S (Cell Signaling) Lef1 Rb 1:400 MA5-14966 (Invitrogen) Laminin Rb 1:400 PA1-16730 (Invitrogen) Mmp14 Rb 1:250 PA5-13183 (Invitrogen) Ecad Rt 1:200 13-1900 (Invitrogen) dpErk Rb 1:100 4377T (Cell Signaling) P-Histone H3 Rb 1:500 441190G (Invitrogen) Foxa2 Rb 1:200 8186S (Cell Signalling) Eomes Rt 1:200 53-4875-80 (Invitrogen) Syndecan-1 Rb 1:300 PA516918 (Invitrogen) HS Chain 10E4 Ms 1:500 Clone F58, 37255-5 (Amsbio) ERK1/2 (Western Blot) Rb 1:1000 4695 (Cell Signalling) pERK (Western Blot) Rb 1:1000 9101 (Cell Signalling) Lamin B Ms 1:1000 ab8982 (Abcam) MitoTracker Deep Red - 1:800 M7512 (Invitrogen) TMRM - 1:800 T668 (Invitrogen) DAPI 405 1:500 D3571 F-Actin (647) 647 1:200 A22287 F-Actin (488) 488 1:500 A12379 donkey anti-mouse Alexa Fluor 488 488 1:500 A21202 donkey anti-rabbit Alexa Fluor 488 488 1:500 A21206 donkey anti-rat Alexa Fluor 488 488 1:500 A21208 donkey anti-mouse Alexa Fluor 568 568 1:500 A10039 donkey anti-goat Alexa Fluor 568 568 1:500 A11057 donkey anti-rabbit Alexa Fluor 568 568 1:500 A10042 donkey anti-mouse Alexa Fluor 647 647 1:500 A31571 donkey anti-goat Alexa Fluor 647 647 1:500 A21447 donkey anti-rabbit Alexa Fluor 647 647 1:500 A31573 Anti-rabbit IgG, HRP-linked Antibody HRP 1:5000 7074 Anti-mouse IgG, HRP-linked Antibody HRP 1:5000 7076 Validation The subcellular localization of all the proteins analyzed in this study has been previously reported in mouse embryo (shown as immunofluorescence labelling). .. Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) The ERK-KTR mESCs used in this study source from Simon et al (JAX#035333).
Immunofluorescence:Article Title: Selective utilization of glucose metabolism guides mammalian gastrulation
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Glut1 Ms 1:250 MA1-37783 (Invitrogen) Snai1 Gt 1:200 AF3639 (R&D Systems) Glut3 Rb 1:250 MA532696 (Invitrogen) NCad Ms 1:200 MA1-91128 (Invitrogen) Sox2 Rt 1:800 14-9811-82 (Invitrogen) T/Bra Gt 1:500, 1:200 AF2085 (R&D Systems) T/Bra Rb 1:250 81694S (Cell Signaling) Lef1 Rb 1:400 MA5-14966 (Invitrogen) Laminin Rb 1:400 PA1-16730 (Invitrogen) Mmp14 Rb 1:250 PA5-13183 (Invitrogen) Ecad Rt 1:200 13-1900 (Invitrogen) dpErk Rb 1:100 4377T (Cell Signaling) P-Histone H3 Rb 1:500 441190G (Invitrogen) Foxa2 Rb 1:200 8186S (Cell Signalling) Eomes Rt 1:200 53-4875-80 (Invitrogen) Syndecan-1 Rb 1:300 PA516918 (Invitrogen) HS Chain 10E4 Ms 1:500 Clone F58, 37255-5 (Amsbio) ERK1/2 (Western Blot) Rb 1:1000 4695 (Cell Signalling) pERK (Western Blot) Rb 1:1000 9101 (Cell Signalling) Lamin B Ms 1:1000 ab8982 (Abcam) MitoTracker Deep Red - 1:800 M7512 (Invitrogen) TMRM - 1:800 T668 (Invitrogen) DAPI 405 1:500 D3571 F-Actin (647) 647 1:200 A22287 F-Actin (488) 488 1:500 A12379 donkey anti-mouse Alexa Fluor 488 488 1:500 A21202 donkey anti-rabbit Alexa Fluor 488 488 1:500 A21206 donkey anti-rat Alexa Fluor 488 488 1:500 A21208 donkey anti-mouse Alexa Fluor 568 568 1:500 A10039 donkey anti-goat Alexa Fluor 568 568 1:500 A11057 donkey anti-rabbit Alexa Fluor 568 568 1:500 A10042 donkey anti-mouse Alexa Fluor 647 647 1:500 A31571 donkey anti-goat Alexa Fluor 647 647 1:500 A21447 donkey anti-rabbit Alexa Fluor 647 647 1:500 A31573 Anti-rabbit IgG, HRP-linked Antibody HRP 1:5000 7074 Anti-mouse IgG, HRP-linked Antibody HRP 1:5000 7076 Validation The subcellular localization of all the proteins analyzed in this study has been previously reported in mouse embryo (shown as immunofluorescence labelling). .. Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) The ERK-KTR mESCs used in this study source from Simon et al (JAX#035333).
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